USE OF BACTERIA IN RECLAIMING SOIL CONTAMINATED WITH HERBICIDES 2, 4 DINITROPHENYLACETATE AND 4 – CHLOROPHENYL ETHANAMIDE MIXTURE IN SOIL

Patrick O., Atusa K.
Publisher: A MULTI-DISCIPLINARY JOURNAL OF THE FEDERAL COLLEGE OF EDUCATION (TECHNICAL), UMUNZE, ANAMBRA STATE {"id":6,"volume_name":"Vol 5 Number 2","created_at":null,"updated_at":null,"url":"volumes\/5.jpg"} (2): 1118-0315: doi: January 2017 Published in 2017

An investigation was carried out in search for reclamations of contamination soils using herbicide degrading bacteria. Soil samples were taken from rice farm contaminated with the herbicides solution using mineral salt agar (MSA) without herbicides solution as control. Screening involved purification by cloning three successive times in herbicide supplemented MSA and isolation of pure and subsequent subculture in herbicide supplemented mineral salt broth (MSB). Bacteria growth was determined by colony formation on MSA as well as by optical density (OD) of MSB cultured at 600mm using spectronic – 20. Out of the bacteria strain isolated from these herbicide contaminated soils only one organism showed positive herbicide utilization ability as sole carbon and energy source. Pure cultures of this isolate was characterized using standard technological techniques and identified as Pseudomonas sp. It is therefore recommended that herbicides that are biodegradable, such as synthesized herbicides should be used for weeds control in the farm.

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